The DoD SIOO conducts vigorous and independent inspections of DoD intelligence activities worldwide, ranging from intelligence staffs at strategic headquarters to tactical intelligence activities in the field. DoD SIOO inspects all intelligence disciplines e.g. Human Intelligence, Imagery Intelligence, Signals Intelligence, Measurement and Signatures Intelligence, as well as Counterintelligence. DoD SIOO verifies that personnel are trained in and comply with EO 12333 and DoD implementing authorities, and that they are familiar with their obligation to report questionable or improper intelligence activities.

The DoD SIOO is also responsible for determining the effectiveness of the Intelligence Oversight programs of the entire DoD intelligence community. To accomplish this task, the inspection programs of the Inspectors General of the Joint Staff, Military Services, Combatant Commands, and DoD intelligence agencies are monitored.